Yin Ruixing, Pan Shangling, Wu Jinzhen, Lin Weixiong, Yang Dezhai
Department of Cardiology, Institute of Cardiovascular Diseases, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, P. R. China.
Exp Biol Med (Maywood). 2008 Apr;233(4):409-18. doi: 10.3181/0709-RM-254.
Hei Yi Zhuang is an isolated subgroup of the Zhuang minority in China. Little is known about the distribution of apolipoprotein (apo) E genetic variations and its role in lipid metabolism in this population. The present study was undertaken to compare the effect of apoE gene polymorphism on serum lipid levels between the Guangxi Hei Yi Zhuang and Han populations. A total of 873 subjects of Hei Yi Zhuang and 867 participants of Han Chinese were surveyed by a stratified randomized cluster sampling. Genotyping of apoE was performed using polymerase chain reaction and restriction fragment length polymorphism. The frequencies of 2, 3, and 4 alleles were 15.23%, 79.84%, and 4.93% in Hei Yi Zhuang, and 9.23%, 81.43%, and 9.34% in Han (P < 0.001); respectively. The frequencies of 2/ 2, 2/ 3, 2/ 4, 3/ 3, 3/ 4, and 4/ 4 genotypes were 4.70%, 17.86%, 3.21%, 68.16%, 5.50%, and 0.57% in Hei Yi Zhuang, and 2.54%, 9.23%, 4.15%, 70.70%, 12.23%, and 1.15% in Han (P < 0.001); respectively. Total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and apoB levels were lower in Hei Yi Zhuang than in Han (P < 0.01-0.001), but high-density lipoprotein cholesterol (HDL-C) levels and the ratio of apoA-I to apoB were higher in Hei Yi Zhuang than in Han (P < 0.001 for each). There were significant differences in TC, HDL-C, LDL-C, and apoB levels among the six genotypes in both ethnic groups (P < 0.01-0.001). Hyperlipidemia was positively correlated with age, body mass index, hypertension, alcohol consumption, and apoE allele in both populations (P < 0.05-0.001). TC, LDL-C, and apoB levels were positively correlated, and HDL-C levels were negatively associated with apoE genotypes in both ethnic groups (P < 0.001 for all). The differences in the lipid profiles between Hei Yi Zhuang and Han Chinese might partly attribute to the differences in apoE genotypic and allelic frequencies.
黑衣壮是中国壮族的一个孤立分支群体。目前对于该群体载脂蛋白（apo）E基因变异的分布及其在脂质代谢中的作用了解甚少。本研究旨在比较广西黑衣壮和汉族人群中apoE基因多态性对血脂水平的影响。采用分层随机整群抽样方法，对873名黑衣壮受试者和867名汉族参与者进行了调查。使用聚合酶链反应和限制性片段长度多态性方法对apoE进行基因分型。黑衣壮人群中2、3和4等位基因的频率分别为15.23%、79.84%和4.93%，汉族人群中分别为9.23%、81.43%和9.34%（P<0.001）。黑衣壮人群中2/2、2/3、2/4、3/3、3/4和4/4基因型的频率分别为4.70%、17.86%、3.21%、68.16%、5.50%和0.57%，汉族人群中分别为2.54%、9.23%、4.15%、70.70%、12.23%和1.15%（P<0.001）。黑衣壮人群的总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）和apoB水平低于汉族（P<0.01 - 0.001），但高密度脂蛋白胆固醇（HDL-C）水平以及apoA-I与apoB的比值高于汉族（各P<0.001）。两个民族群体的六种基因型在TC、HDL-C、LDL-C和apoB水平上均存在显著差异（P<0.01 - 0.001）。两个群体中高脂血症均与年龄、体重指数、高血压、饮酒和apoE等位基因呈正相关（P<0.05 - 0.001）。两个民族群体中，TC、LDL-C和apoB水平呈正相关，HDL-C水平与apoE基因型呈负相关（均P<0.001）。黑衣壮和汉族人群血脂谱的差异可能部分归因于apoE基因型和等位基因频率的差异。
