广西黑衣壮族和汉族人群中脂蛋白脂肪酶基因PvuII位点多态性与血脂水平的关系

Lipoprotein lipase gene polymorphism at the PvuII locus and serum lipid levels in Guangxi Hei Yi Zhuang and Han populations.

作者信息

Yin Ruixing, Wang Yong, Chen Guangqin, Lin Weixiong, Yang Dezhai, Pan Shangling

机构信息

Department of Cardiology, Institute of Cardiovascular Diseases, First Affiliated Hospital, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, China.

出版信息

Clin Chem Lab Med. 2006;44(12):1416-21. doi: 10.1515/CCLM.2006.273.

DOI:10.1515/CCLM.2006.273

PMID:17163816

Abstract

BACKGROUND

Hei Yi (which means "black worship" and "black dressing") Zhuang is a specific subgroup of the Zhuang nationality in China. Little is known about the relationship between genetic factors and lipid profiles in this population. Therefore, the present study was undertaken to compare the effects of lipoprotein lipase gene polymorphism at the PvuII locus on lipid levels in the Guangxi Hei Yi Zhuang and Han populations.

METHODS

A total of 325 Hei Yi Zhuang subjects aged from 20 to 80 years were surveyed using stratified randomized cluster sampling. Serum levels of lipids and apolipoproteins were measured. Gene polymorphism was determined using polymerase chain reaction and restriction fragment length polymorphism. The results were compared with those for 331 matched Han subjects living in the same district.

RESULTS

Serum levels of total cholesterol, triglycerides, low-density lipoprotein cholesterol and apolipoprotein B were significantly lower in Hei Yi Zhuang than in Han subjects (p<0.05-0.01), whereas the levels of high-density lipoprotein cholesterol and the ratio of apolipoprotein A1 to apolipoprotein B were significantly higher in Hei Yi Zhuang than in Han subjects (both p<0.01). The allelic frequencies for P+ and P- were 52.92% and 47.08% in Hei Yi Zhuang, and 58.46% and 41.54% in Han subjects (p<0.05), respectively. The frequencies of P+P+, P+P- and P-P- genotypes were 23.08%, 59.69% and 17.23% in Hei Yi Zhuang, and 29.31%, 58.31% and 12.38% in Han subjects (p>0.05), respectively. There were no significant differences or no significant correlation between serum lipid parameters and genotypes in Hei Yi Zhuang or Han subjects, or for the combined population of Hei Yi Zhuang and Han (all p>0.05).

CONCLUSIONS

The allelic frequencies of the lipoprotein lipase gene at the PvuII locus in Hei Yi Zhuang were different from those in Han subjects, but the genotypic frequencies in Hei Yi Zhuang subjects were not different from those in Han subjects. There was no significant correlation between polymorphism of the lipoprotein lipase gene at the PvuII site and serum lipid levels in the two ethnic groups.

摘要

背景

黑衣壮是中国壮族的一个特殊分支（“黑衣”意为“崇尚黑色”和“身着黑衣”）。关于该人群遗传因素与血脂谱之间的关系，人们了解甚少。因此，本研究旨在比较广西黑衣壮和汉族人群中脂蛋白脂肪酶基因PvuII位点多态性对血脂水平的影响。

方法

采用分层随机整群抽样法对325名年龄在20至80岁之间的黑衣壮受试者进行调查。检测血清脂质和载脂蛋白水平。采用聚合酶链反应和限制性片段长度多态性方法测定基因多态性。将结果与居住在同一地区的331名匹配汉族受试者的结果进行比较。

结果

黑衣壮受试者的血清总胆固醇、甘油三酯、低密度脂蛋白胆固醇和载脂蛋白B水平显著低于汉族受试者（p<0.05 - 0.01），而黑衣壮受试者的高密度脂蛋白胆固醇水平以及载脂蛋白A1与载脂蛋白B的比值显著高于汉族受试者（均p<0.01）。黑衣壮人群中P+和P-的等位基因频率分别为52.92%和47.08%，汉族受试者中分别为58.46%和41.54%（p<0.05）。黑衣壮人群中P+P+、P+P-和P-P-基因型频率分别为23.08%、59.69%和17.23%，汉族受试者中分别为29.31%、58.31%和12.38%（p>0.05）。黑衣壮或汉族受试者以及黑衣壮和汉族的合并人群中，血清脂质参数与基因型之间均无显著差异或显著相关性（均p>0.05）。