广西黑衣壮族和汉族人群中胆固醇酯转运蛋白基因TaqIB多态性与血脂水平的关联

Association of TaqIB polymorphism in the cholesteryl ester transfer protein gene with serum lipid levels in the Guangxi Hei Yi Zhuang and Han populations.

作者信息

Yijiang Zhou, Ruixing Yin, Yiyang Li, Yaju Deng, Shangling Pan, Weixiong Lin, Dezhai Yang

机构信息

Department of Cardiology, Institute of Cardiovascular Diseases, the First Affiliated Hospital, People's Republic of China.

出版信息

J Investig Med. 2008 Aug;56(6):847-57. doi: 10.2310/JIM.0b013e3181820832.

DOI:10.2310/JIM.0b013e3181820832

PMID:18667902

Abstract

BACKGROUND

Cholesteryl ester transfer protein (CETP) plays an important role in lipoprotein metabolism. The present study was undertaken to compare the difference in the CETP TaqIB gene polymorphism and its association with serum lipid levels between the Guangxi Hei Yi Zhuang and Han populations.

METHODS

A total of 758 subjects of Hei Yi Zhuang and 778 participants of Han Chinese were surveyed. Genotyping of the CETP TaqIB was performed using polymerase chain reaction and restriction fragment length polymorphism and then confirmed using direct sequencing.

RESULTS

The genotypic and allelic frequencies were significant differences between smokers and nonsmokers, or between hypertensives and normotensives in Hei Yi Zhuang, and between drinkers and nondrinkers in Han. The levels of high-density lipoprotein cholesterol (HDL-C) and apolipoprotein AI in Hei Yi Zhuang but not in Han were higher in B2B2 genotype than in B1B1 genotype (P < 0.01 for each). Higher HDL-C levels in Hei Yi Zhuang were found only in females, nondrinkers, nonsmokers, subjects with a body mass index of 24 kg/m or lesser, or normotensives in B2B2 genotype. Higher HDL-C levels in Han were found only in females in B2B2 genotype and in subjects with a body mass index of 24 kg/m or lesser or normotensives in B1B2 genotype. The levels of HDL-C in B1B1 and B1B2 individuals in both ethnic groups were higher in drinkers than in nondrinkers.

CONCLUSIONS

There were significant differences in the interactions between the CETP TaqIB genotypes and several environmental factors in the Hei Yi Zhuang and Han populations. The polymorphism predicted differences in HDL-C and ApoAI in the Hei Yi Zhuang but not in the Han Chinese, even after adjustment for confounding variables. This means that the gene may not be truly involved in regulation of high-density lipoprotein metabolism or that there is an ethnic-specific effect.

摘要

背景

胆固醇酯转运蛋白（CETP）在脂蛋白代谢中起重要作用。本研究旨在比较广西黑衣壮族和汉族人群中CETP TaqIB基因多态性的差异及其与血脂水平的关联。

方法

共调查了758名黑衣壮族受试者和778名汉族参与者。采用聚合酶链反应和限制性片段长度多态性方法对CETP TaqIB进行基因分型，然后用直接测序法进行验证。

结果

黑衣壮族吸烟者与非吸烟者、高血压患者与血压正常者之间，以及汉族饮酒者与非饮酒者之间的基因型和等位基因频率存在显著差异。黑衣壮族B2B2基因型的高密度脂蛋白胆固醇（HDL-C）和载脂蛋白AI水平高于B1B1基因型，而汉族则不然（每项P<0.01）。仅在黑衣壮族中，B2B2基因型的女性、非饮酒者、非吸烟者、体重指数为24kg/m²或更低者或血压正常者的HDL-C水平较高。仅在汉族中，B2B2基因型的女性、体重指数为24kg/m²或更低者或B1B2基因型的血压正常者的HDL-C水平较高。两个民族中B1B1和B1B2个体的HDL-C水平在饮酒者中高于非饮酒者。