Rocha Bruno Aragão, Negro Gilda Maria Barbaro del, Yamamoto Lidia, Souza Mariana Vitule Brito de, Precioso Alexander Roberto, Okay Thelma Suely
Laboratory of Medical Investigation, Department of Pediatrics, Faculdade de Medicina, Children's Institute, Universidade de São Paulo, Cerqueira César, São Paulo, SP, Brazil.
Rev Soc Bras Med Trop. 2008 Jan-Feb;41(1):1-5. doi: 10.1590/s0037-86822008000100001.
Thirty-four Candida isolates were analyzed by random amplified polymorphic DNA using the primer OPG-10:24 Candida albicans; 4 Candida tropicalis; 2 Candida parapsilosis; 2 Candida dubliniensis; 1 Candida glabrata and 1 Candida krusei. The UPGMA-Pearson correlation coefficient was used to calculate the genetic distance between the different Candida groupings. Samples were classified as identical (correlation of 100%); highly related samples (90%); moderately related samples (80%) and unrelated samples (< 70%). The results showed that the RAPD proposed was capable of classifying the isolates coherently (such that same species were in the same dendrogram), except for two isolates of Candida parapsilosis and the positive control (Netherlands, 1973), probably because they are now recognized as three different species. Concerning the only fluconazole-resistant Candida tropicalis isolate with a genotype that was different to the others, the data were insufficient to affirm that the only difference was the sensitivity to fluconazole. We concluded that the Random Amplified Polymorphic DNA proposed might be used to confirm Candida species identified by microbiological methods.
使用引物OPG - 10通过随机扩增多态性DNA对34株念珠菌分离株进行了分析：24株白色念珠菌；4株热带念珠菌；2株近平滑念珠菌；2株都柏林念珠菌；1株光滑念珠菌和1株克柔念珠菌。采用UPGMA - 皮尔逊相关系数计算不同念珠菌分组之间的遗传距离。样本被分类为相同（相关性为100%）；高度相关样本（90%）；中度相关样本（80%）和不相关样本（<70%）。结果表明，除了两株近平滑念珠菌分离株和阳性对照（荷兰，1973）外，所提出的RAPD能够连贯地对分离株进行分类（即同一物种在同一聚类图中），可能是因为它们现在被认为是三个不同的物种。关于唯一一株对氟康唑耐药的热带念珠菌分离株，其基因型与其他菌株不同，数据不足以肯定唯一的差异就是对氟康唑的敏感性。我们得出结论，所提出的随机扩增多态性DNA可能用于确认通过微生物学方法鉴定的念珠菌物种。
