Galli Rossella, Gritti Angela, Vescovi Angelo L
Stem Cell Research Institute (HSR-SCRI), DIBI-H.S. Raffaele, Milan, Italy.
Methods Mol Biol. 2008;438:67-84. doi: 10.1007/978-1-59745-133-8_7.
Neural stem cells (NSCs) have been identified in the mature central nervous system (CNS), and they reside in specific areas. Cultures of NSCs can be successfully established in vitro by exploiting the NeuroSphere assay. This methodology relies on the continuous exposure of neural cells to mitogens such as epidermal growth factor and fibroblast growth factor-2. Under these conditions, only NSCs and highly undifferentiated progenitors proliferate, whereas committed precursors and terminally differentiated cells are eliminated from the culture. The proper application of this method to the cells allows the establishment of long-term expanding stable NSC lines, starting from different neural tissues as the adult rodent CNS and human brain tumor specimens.
神经干细胞（NSCs）已在成熟的中枢神经系统（CNS）中被鉴定出来，它们存在于特定区域。通过利用神经球检测法，可以在体外成功建立神经干细胞培养体系。该方法依赖于神经细胞持续暴露于有丝分裂原，如表皮生长因子和成纤维细胞生长因子-2。在这些条件下，只有神经干细胞和高度未分化的祖细胞能够增殖，而定向祖细胞和终末分化细胞则从培养物中被清除。将该方法正确应用于细胞，能够从不同的神经组织（如成年啮齿动物的中枢神经系统和人脑肿瘤标本）开始，建立长期扩增的稳定神经干细胞系。
