Sauer Michael, Friml Jirí
ZMBP Developmental Genetics, University of Tübingen, Germany.
Methods Mol Biol. 2008;427:71-6. doi: 10.1007/978-1-59745-273-1_5.
Embryogenesis of Arabidopsis thaliana follows a nearly invariant cell division pattern and provides an ideal system for studies of early plant development. However, experimental manipulation with embryogenesis is difficult, as the embryo develops deeply inside maternal tissues. Here, we present a method to culture zygotic Arabidopsis embryos in vitro. It enables culturing for prolonged periods of time from the first developmental stages on. The technique omits excision of the embryo by culturing the entire ovule, which facilitates the manual procedure. It allows pharmacological manipulation of embryo development and does not interfere with standard techniques for localizing gene expression and protein localization in the cultivated embryos.
拟南芥的胚胎发生遵循几乎不变的细胞分裂模式，为早期植物发育研究提供了一个理想的系统。然而，由于胚胎在母体组织内部深处发育，对胚胎发生进行实验操作很困难。在这里，我们提出了一种在体外培养拟南芥合子胚的方法。它能够从最初的发育阶段开始进行长时间培养。该技术通过培养整个胚珠而省略了胚胎的切除步骤，这简化了人工操作过程。它允许对胚胎发育进行药理学操作，并且不会干扰在培养胚胎中定位基因表达和蛋白质定位的标准技术。
