Crittenden Sarah L, Kimble Judith
Howard Hughes Medical Institute and Department of Biochemistry, University of Wisconsin Madison, Madison, WI, USA.
Methods Mol Biol. 2008;450:27-44. doi: 10.1007/978-1-60327-214-8_2.
We present methods for characterizing the mitotic and early meiotic regions of the Caenorhabditis elegans germline. The methods include examination of germlines in living and fixed worms, cell cycle analysis, analysis of markers, and initial characterization of mutants that affect germline proliferation.
我们展示了用于表征秀丽隐杆线虫生殖系有丝分裂和减数分裂早期区域的方法。这些方法包括检查活虫和固定虫中的生殖系、细胞周期分析、标记物分析以及对影响生殖系增殖的突变体的初步表征。