Pitaru S, Hekmati M, Metzger Z, Savion N
Section of Oral Biology, Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Israel.
J Periodontal Res. 1991 Nov;26(6):461-7. doi: 10.1111/j.1600-0765.1991.tb01796.x.
In the present study, and in vitro system was developed and designed to examine the interaction between gingival fibroblasts (GF) and epithelial cells (EC) on the tooth surface. Porcine roots were cut transversely into 300 microns-thick root slices (RS). Gingival explants were placed on the upper RS surface and cultured in a defined medium permissive for the growth of EC. After 4 or 6 days, RS yielding EC were transferred onto confluent cultures of GF and further co-cultured for either 4 or 8 d. Cultures were then fixed and examined by SEM. The upper RS surfaces and the upper half of their peripheral aspect were covered by EC. The lower half of the peripheral RS surfaces were populated by GF originating from the confluent culture of GF. EC and GF made contact at approximately the middle of the side of the root slice. In cultures of epithelial components grown in defined medium for either 4 or 6 d and harvested 4 d after assembling the system, the EC-GF junction was located 117 +/- 45 and 271 +/- 82 microns, respectively from the upper RS aspect. Extending the co-culture period did not affect the EC-GF junction location. These results indicate that GF-EC contact stops the migration of these cells on root surfaces in vitro. The described system should be valuable for studying cellular events that may affect the formation of a new dentogingival junction following surgical periodontal therapy.
在本研究中，开发并设计了一种体外系统，以检查牙龈成纤维细胞（GF）与牙齿表面上皮细胞（EC）之间的相互作用。将猪牙根横向切成300微米厚的根切片（RS）。将牙龈外植体置于RS上表面，并在允许EC生长的特定培养基中培养。4或6天后，产生EC的RS转移到GF的汇合培养物上，并进一步共培养4或8天。然后将培养物固定并用扫描电子显微镜检查。RS上表面及其周边部分的上半部分被EC覆盖。RS周边表面的下半部分由源自GF汇合培养物的GF占据。EC和GF在根切片侧面的大约中间位置接触。在特定培养基中生长4或6天并在组装系统后4天收获的上皮成分培养物中，EC-GF连接分别距离RS上表面117±45和271±82微米。延长共培养时间不影响EC-GF连接位置。这些结果表明，GF-EC接触会阻止这些细胞在体外根表面的迁移。所描述的系统对于研究可能影响牙周手术治疗后新牙-龈结合形成的细胞事件应该是有价值的。
