Pitaru S, Hekmati M, Geiger S, Savion N
Department of Oral Biology, Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Israel.
J Dent Res. 1988 Nov;67(11):1386-91. doi: 10.1177/00220345880670110501.
The capacity of mineralized cementum to support epithelial cell migration and growth and the effect that fibronectin and partial demineralization of cementum have on these processes were assessed in vitro. Dog gingival explants, 1 X 2 mm, were cultured on the cementum surfaces of pig root pieces in a defined medium consisting of DMEM and F12 (1V/1V), transferrin, insulin, epidermal growth factor, cortisone, selenium, and high-density lipoprotein. Sixty root pieces were divided into four equal groups according to the treatment: (1) untreated mineralized cementum; (2) treated with 5 micrograms of fibronectin; (3) partially demineralized in 18% EDTA for 30 min; and (4) both partially demineralized and fibronectin-treated as above. Epithelial cell migration and growth on each of the four differently treated cementum surfaces were assessed histomorphometrically by means of scanning electron microscopy. The defined culture medium supported the selective migration and growth of epithelial cells from the gingival explants onto the mineralized cementum. This was confirmed by the positive immunostaining of these cells with antikeratin antibodies. Partial demineralization of cementum inhibited epithelial migration and growth by 83% and 91%, respectively. Fibronectin treatment did not affect epithelial cell migration and growth on mineralized cementum, but it decreased the degree of epithelial cell migration and growth inhibition on partially demineralized cementum to 57% and 43%, respectively. The results indicate that: (i) mineralized cementum may consist of components that are recognized by gingival epithelial cells and support their migration and growth in vitro; (ii) these components can be removed by demineralization; and (iii) fibronectin partially restores epithelial cell migration and growth on partial demineralized cementum in vitro.
在体外评估了矿化牙骨质支持上皮细胞迁移和生长的能力，以及纤连蛋白和牙骨质部分脱矿对这些过程的影响。将1×2毫米的犬牙龈外植体培养在猪牙根片的牙骨质表面，培养基为含有DMEM和F12（1:1体积比）、转铁蛋白、胰岛素、表皮生长因子、可的松、硒和高密度脂蛋白的限定培养基。根据处理方式将60个牙根片分为四组：（1）未处理的矿化牙骨质；（2）用5微克纤连蛋白处理；（3）在18%乙二胺四乙酸（EDTA）中部分脱矿30分钟；（4）如上述同时进行部分脱矿和纤连蛋白处理。通过扫描电子显微镜以组织形态计量学方法评估上皮细胞在四种不同处理的牙骨质表面上的迁移和生长。限定培养基支持上皮细胞从牙龈外植体选择性迁移并生长到矿化牙骨质上。用抗角蛋白抗体对这些细胞进行阳性免疫染色证实了这一点。牙骨质部分脱矿分别抑制上皮细胞迁移和生长83%和91%。纤连蛋白处理对矿化牙骨质上的上皮细胞迁移和生长没有影响，但它将部分脱矿牙骨质上上皮细胞迁移和生长的抑制程度分别降至57%和43%。结果表明：（i）矿化牙骨质可能由牙龈上皮细胞识别并在体外支持其迁移和生长的成分组成；（ii）这些成分可通过脱矿去除；（iii）纤连蛋白在体外部分恢复了部分脱矿牙骨质上的上皮细胞迁移和生长。
