Asiimwe B B, Koivula T, Källenius G, Huard R C, Ghebremichael S, Asiimwe J, Joloba M L
Department of Medical Microbiology, Makerere University Medical School, Kampala, Uganda.
Int J Tuberc Lung Dis. 2008 Apr;12(4):386-91.
Rubaga Division, Kampala, Uganda.
To use polymerase chain reaction (PCR) based regions of difference (RD) analysis to study the species diversity of Mycobacterium tuberculosis complex isolates from a community-based sample of tuberculosis (TB) patients from Rubaga and to perform long sequence polymorphism (LSP) analysis to further characterise the M. tuberculosis Uganda genotype, a group of strains previously recognised by their characteristic spoligotype patterns.
For the present study, 344 consecutive TB patients attending clinics in Rubaga Division were enrolled. Sample processing and culture were performed at the National Tuberculosis and Reference Laboratory and molecular assays at Makerere Medical School. Species identification was achieved by determining the RDs, while spoligotyping and LSP analysis were performed to characterise the M. tuberculosis Uganda genotype.
Of the 344 isolates, 343 (99.7%) were M. tuberculosis sensu stricto, while one was classical M. bovis. The Uganda genotype strains characteristically lacked RD724, a locus that defines one of the major sub-lineages of M. tuberculosis, which suggested that this geographically constrained lineage is specifically adapted to a central African human host population.
M. tuberculosis is the most prevalent species of the M. tuberculosis complex in Kampala, and the Uganda genotype is the predominant strain.
乌干达坎帕拉鲁巴加区。
运用基于聚合酶链反应（PCR）的差异区域（RD）分析，研究来自鲁巴加社区结核病（TB）患者样本中结核分枝杆菌复合群分离株的物种多样性，并进行长序列多态性（LSP）分析，以进一步鉴定结核分枝杆菌乌干达基因型，这是一组先前因其特征性的间隔寡核苷酸分型模式而被识别的菌株。
在本研究中，纳入了344名连续就诊于鲁巴加区诊所的结核病患者。样本处理和培养在国家结核病和参考实验室进行，分子检测在马凯雷雷医学院进行。通过确定RDs进行物种鉴定，同时进行间隔寡核苷酸分型和LSP分析以鉴定结核分枝杆菌乌干达基因型。
在344株分离株中，343株（99.7%）为狭义结核分枝杆菌，1株为经典牛分枝杆菌。乌干达基因型菌株的特征是缺乏RD724，该位点定义了结核分枝杆菌的一个主要亚谱系，这表明这个地理上受限的谱系特别适应中非人类宿主群体。
结核分枝杆菌是坎帕拉结核分枝杆菌复合群中最常见的物种，乌干达基因型是主要菌株。
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