贝宁结核病的首次分子流行病学研究。
First molecular epidemiological study of tuberculosis in Benin.
作者信息
Affolabi D, Anyo G, Faïhun F, Sanoussi N, Shamputa I C, Rigouts L, Kestens L, Anagonou S, Portaels F
机构信息
Laboratoire de Référence des Mycobactéries, Cotonou, Bénin; Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium.
出版信息
Int J Tuberc Lung Dis. 2009 Mar;13(3):317-22.
OBJECTIVES
To assess the diversity of Mycobacterium tuberculosis strains in Cotonou, Benin, and the risk factors associated with clustering.
METHODS
We analysed one sputum sample from 194 consecutive new pulmonary tuberculosis (TB) cases using two genotyping methods: spoligotyping and the 12 loci mycobacterial interspersed repetitive unit-variable number of tandem repeats (MIRU-VNTR). The data obtained were compared to the SpolDB4.0 database.
RESULTS
We have found that spoligotype 61, highly predominant in West Africa, was also the most prevalent strain in Cotonou. We observed that the Beijing family represented 10.3% of strains and was associated with resistance to streptomycin. We also confirmed that combining spoligotyping and MIRU-VNTR provided a higher discriminatory power than the two techniques used individually.
CONCLUSION
Spoligotype 61 and Beijing genotype are the most prevalent genotypes of M. tuberculosis in Cotonou.
目的
评估贝宁科托努结核分枝杆菌菌株的多样性以及与聚集相关的危险因素。
方法
我们使用两种基因分型方法对194例连续新发肺结核病例的一份痰标本进行分析：间隔寡核苷酸分型法（spoligotyping）和12个位点的分枝杆菌插入重复单位可变数目串联重复序列（MIRU-VNTR）。将获得的数据与SpolDB4.0数据库进行比较。
结果
我们发现，在西非高度占优势的间隔寡核苷酸分型61型，也是科托努最流行的菌株。我们观察到北京家族菌株占菌株总数的10.3%，且与链霉素耐药相关。我们还证实，联合使用间隔寡核苷酸分型法和MIRU-VNTR比单独使用这两种技术具有更高的鉴别力。
结论
间隔寡核苷酸分型61型和北京基因型是科托努结核分枝杆菌最流行的基因型。
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