Singh Shree Ram, Liu Wei, Hou Steven X
The Mouse Cancer Genetics Program, National Cancer Institute at Frederick, National Institutes of Health, Frederick, MD 21702, USA.
Cell Stem Cell. 2007 Aug 16;1(2):191-203. doi: 10.1016/j.stem.2007.07.003.
All animals must excrete the waste products of metabolism. Excretion is performed by the kidney in vertebrates and by the Malpighian tubules in Drosophila. The mammalian kidney has an inherent ability for recovery and regeneration after ischemic injury. Stem cells and progenitor cells have been proposed to be responsible for repair and regeneration of injured renal tissue. In Drosophila, the Malpighian tubules are thought to be very stable and no stem cells have been identified. We have identified multipotent stem cells in the region of lower tubules and ureters of the Malpighian tubules. Using lineage tracing and molecular marker labeling, we demonstrated that several differentiated cells in the Malpighian tubules arise from the stem cells and an autocrine JAK-STAT signaling regulates the stem cells' self-renewal. Identifying adult kidney stem cells in Drosophila may provide important clues for understanding mammalian kidney repair and regeneration during injury.
所有动物都必须排泄新陈代谢的废物。在脊椎动物中,排泄由肾脏完成;在果蝇中,排泄由马氏管完成。哺乳动物的肾脏在缺血性损伤后具有内在的恢复和再生能力。干细胞和祖细胞被认为负责受损肾组织的修复和再生。在果蝇中,马氏管被认为非常稳定,尚未发现干细胞。我们在马氏管的下小管和输尿管区域鉴定出了多能干细胞。通过谱系追踪和分子标记物标记,我们证明马氏管中的几种分化细胞源自干细胞,并且自分泌的JAK-STAT信号调节干细胞的自我更新。在果蝇中鉴定成年肾脏干细胞可能为理解哺乳动物肾脏损伤后的修复和再生提供重要线索。