Xu Xiao-Qin, Hu Guang-Lin, Shen Jin-Can, Li Qi, Wang Xiao-Ru
The Key Laboratory of Analytical Sciences of Ministry of Education of China, College of Chemistry and Chemical Engineering, Xiamen Unitersity, Xiamen 361005, China.
Se Pu. 2002 Sep;20(5):446-8.
A simple and accurate method for the determination of andrographolide and dehydroandrographolide in andrographis paniculata Nees materials and patent medicines with high performance liquid chromatography (HPLC) has been developed. The two components were extracted from powdered samples by shaking with methanol. The resultant extracts were separated within 15 min on a BECKMAN C18 column (4.6 mm i. d. x 250 mm, 5 microm) and with a gradient elution of acetonitrile-water at a flow rate of 0.5 mL/min. The detection wavelength was 225 nm and the injection volume was 20 microL. In gradient elution program the volume fraction of acetonitrile in mobile phase was as follows: 0 min - 1 min, 40%; 1 min - 5 min, 40% - 50%; 5 min - 15 min, 50% - 70%. Both andrographolide and dehydroandrographolide have good linearity in the range of 10 mg/L to 100 mg/L with the correlation coefficients of 0.997 6 and 0.998 6 respectively. This method has been successfully applied for the analysis of andrographis paniculata Nees materials and related patent medicines.
建立了一种用高效液相色谱（HPLC）测定穿心莲药材及中成药中穿心莲内酯和脱水穿心莲内酯的简单准确方法。将粉末状样品用甲醇振摇提取这两种成分。所得提取物在BECKMAN C18柱（内径4.6 mm×250 mm，5μm）上于15分钟内分离，采用乙腈 - 水梯度洗脱，流速为0.5 mL/min。检测波长为225 nm，进样量为20μL。在梯度洗脱程序中，流动相中乙腈的体积分数如下：0分钟 - 1分钟，40%；1分钟 - 5分钟，40% - 50%；5分钟 - 15分钟，50% - 70%。穿心莲内酯和脱水穿心莲内酯在10 mg/L至100 mg/L范围内均具有良好的线性，相关系数分别为0.997 6和0.998 6。该方法已成功应用于穿心莲药材及相关中成药的分析。
