The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.
Parasitol Res. 2010 Nov;107(6):1489-94. doi: 10.1007/s00436-010-2024-6. Epub 2010 Aug 10.
The distribution and public health significance of Cryptosporidium species/genotypes in pigs differ among geographic areas and studies. To characterize the prevalence of cryptosporidiosis in pigs in Henan, China, a total of 1,350 fecal samples from 14 farms in ten prefectures in Henan Province were examined. The overall prevalence of Cryptosporidium was 8.2% (111/1,350), with the highest infection rate (79/383 or 20.6%) in 1-2-month-old piglets and the lowest infection rates in 3-6-month-old pigs. Cryptosporidium-positive samples from 108 animals were analyzed by polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis of the small subunit rRNA gene, and 35 were further analyzed by DNA sequencing of the PCR products. Two Cryptosporidium species/genotype were identified, including Cryptosporidium suis (94/108) and the Cryptosporidium pig genotype II (14/108). C. suis infection was more common in younger piglets whereas the pig genotype II was relatively common in older pigs. These findings suggest that pigs are not a major source of zoonotic Cryptosporidium in the study area.
在不同的地理区域和研究中，猪体内隐孢子虫种/基因型的分布和公共卫生意义存在差异。为了描述中国河南省猪隐孢子虫病的流行情况，从河南省十个地区的 14 个农场采集了 1350 份粪便样本进行检测。隐孢子虫的总感染率为 8.2%（111/1350），1-2 月龄仔猪的感染率最高（79/383，20.6%），3-6 月龄仔猪的感染率最低。对 108 份来自阳性样本的动物进行了聚合酶链反应（PCR）-限制性片段长度多态性分析的小亚基 rRNA 基因分析，其中 35 份进一步进行了 PCR 产物的 DNA 测序分析。鉴定出两种隐孢子虫种/基因型，包括猪隐孢子虫（94/108）和猪隐孢子虫基因型 II（14/108）。C. suis 感染在较年轻的仔猪中更为常见，而猪基因型 II 在较年长的猪中更为常见。这些发现表明，在研究区域内，猪不是人类隐孢子虫的主要传染源。
