Kameda A, Yoshikawa N, Shiozawa S, Doi K, Nakamura H
Department of Pediatrics, Kobe University Hospital, Japan.
Nephron. 1991;59(4):546-51. doi: 10.1159/000186642.
In order to examine T lymphocyte function in childhood IgA nephropathy, 13 patients and 10 age-matched control subjects were studied. T lymphocyte function was examined in terms of in vitro immunoglobulin synthesis by peripheral blood mononuclear cells (PBMC) and CD4-depleted (suppressor-rich) and CD8-depleted (helper-rich) PBMC in both unstimulated and pokeweed mitogen (PWM) and Staphylococcus aureus Cowan I (SAC) stimulated cultures. T lymphocyte subpopulations were examined by two-color immunofluorescence analysis using Fluorescein-Activated Cell Sorter (FACS). Children with IgA nephropathy showed (1) a significant increase in IgA synthesis by PBMC with or without mitogen stimulation, (2) a significant increase in IgG and IgA synthesis by CD4-depleted (suppressor-rich) PBMC, (3) a significant increase in IgG and IgA synthesis by CD8-depleted (helper-rich) PBMC, and (4) a significant decrease in suppressor-inducer T cells (Leu3a+Leu8+). These results suggest that a decrease in suppressor-inducer T cells, impaired suppressor T cell function and hyperactivity of helper T cell function are responsible for the increase in IgA production in children with IgA nephropathy.
为了研究儿童IgA肾病中T淋巴细胞的功能，对13例患者和10例年龄匹配的对照者进行了研究。通过外周血单个核细胞（PBMC）以及在未刺激和经商陆有丝分裂原（PWM）和金黄色葡萄球菌Cowan I（SAC）刺激培养的CD4缺失（富含抑制细胞）和CD8缺失（富含辅助细胞）的PBMC的体外免疫球蛋白合成来检测T淋巴细胞功能。使用荧光激活细胞分选仪（FACS）通过双色免疫荧光分析检测T淋巴细胞亚群。IgA肾病患儿表现为：（1）无论有无丝裂原刺激，PBMC合成IgA均显著增加；（2）CD4缺失（富含抑制细胞）的PBMC合成IgG和IgA显著增加；（3）CD8缺失（富含辅助细胞）的PBMC合成IgG和IgA显著增加；（4）抑制诱导性T细胞（Leu3a + Leu8 +）显著减少。这些结果表明，抑制诱导性T细胞减少、抑制性T细胞功能受损以及辅助性T细胞功能亢进是导致IgA肾病患儿IgA产生增加的原因。
