食蟹猴补体的研究
Investigation of cynomolgus monkey complement.
作者信息
Xu H, Kitano E, Sato Y, Kobayashi C, Firdawes S, Ishimaru A, Yamamoto A, Kitamura H, Fukuzawa M, Miyagawa S
机构信息
Division of Organ Transplantation, Department of Molecular Therapeutics, Osaka University Graduate School of Medicine, Osaka, Japan.
出版信息
Transplant Proc. 2008 Mar;40(2):607-8. doi: 10.1016/j.transproceed.2008.01.008.
BACKGROUND
The cynomolgus monkey is one of the most popular recipient animals in xenotransplantation experiments. However, studies of the cynomolgus monkey complement are rare. In the present study, based on the study that compared the hemolytic complement titer in cynomolgus monkeys with that in humans, the complement regulatory function of human decay accelerating factor (CD55) in both human and cynomolgus monkey sera was studied.
METHODS
Hemolytic complement titers in cynomolgus monkeys were calculated using the same methods as are used in humans. Next, the complement regulatory function of human DAF (CD55) in cynomolgus monkey serum was studied using porcine endothelial cells (PECs) and human DAF.
RESULTS
Of the complement titers tested, such as CH50, ACH50, C4, C2, and C3, the values were relatively high, except for the C4 titer. Human DAF on the surface of PEC resulted in nearly identical complement regulatory function in the human and cynomolgus monkey sera.
CONCLUSIONS
Human DAF showed nearly the same complement regulatory function in both human and cynomolgus monkey sera.
背景
食蟹猴是异种移植实验中最常用的受体动物之一。然而，关于食蟹猴补体的研究却很少。在本研究中，基于一项比较食蟹猴与人类溶血补体滴度的研究，对人衰变加速因子（CD55）在人和食蟹猴血清中的补体调节功能进行了研究。
方法
采用与人类相同的方法计算食蟹猴的溶血补体滴度。接下来，使用猪内皮细胞（PEC）和人DAF研究人DAF（CD55）在食蟹猴血清中的补体调节功能。
结果
在所检测的补体滴度中，如CH50、ACH50、C4、C2和C3，除C4滴度外，其他值相对较高。PEC表面的人DAF在人和食蟹猴血清中具有几乎相同的补体调节功能。
结论
人DAF在人和食蟹猴血清中表现出几乎相同的补体调节功能。
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