通过酵母中的功能互补鉴定一种新型分枝杆菌3-羟基酰基硫酯脱水酶HtdZ（Rv0130）。
Identification of a novel mycobacterial 3-hydroxyacyl-thioester dehydratase, HtdZ (Rv0130), by functional complementation in yeast.
作者信息
Gurvitz Aner, Hiltunen J Kalervo, Kastaniotis Alexander J
机构信息
Section of Physiology of Lipid Metabolism, Institute of Physiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria.
出版信息
J Bacteriol. 2008 Jun;190(11):4088-90. doi: 10.1128/JB.00016-08. Epub 2008 Mar 28.
Abstract
We report on the identification of Mycobacterium tuberculosis HtdZ (Rv0130), representing a novel 3-hydroxyacyl-thioester dehydratase. HtdZ was picked up by the functional complementation of Saccharomyces cerevisiae htd2Delta cells lacking the dehydratase of mitochondrial type II fatty acid synthase. Mutant cells expressing HtdZ contained dehydratase activity, recovered their respiratory ability, and partially restored de novo lipoic acid synthesis.
摘要
我们报告了结核分枝杆菌HtdZ（Rv0130）的鉴定，它代表一种新型的3-羟基酰基硫酯脱水酶。HtdZ是通过对缺乏线粒体II型脂肪酸合酶脱水酶的酿酒酵母htd2Δ细胞进行功能互补筛选得到的。表达HtdZ的突变细胞具有脱水酶活性，恢复了呼吸能力，并部分恢复了硫辛酸的从头合成。
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