MacLeod D L, Gyles C L
Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph, Canada.
Vet Microbiol. 1991 Nov;29(3-4):309-18. doi: 10.1016/0378-1135(91)90138-6.
Passive transfer of neutralizing antibodies and active immunization with a toxoid of purified Shiga-like toxin II variant (SLT-IIv, edema disease toxin) were used to protect pigs against challenge with SLT-IIv. Six 6-week-old pigs were passively immunized by intraperitoneal administration of an immunoglobulin preparation from porcine antiserum against purified SLT-IIv. Six 6-week-old pigs and twelve 2-week-old pigs were actively immunized by two intramuscular injections of 25 micrograms of SLT-IIv toxoid given 2 weeks apart. The 24 immunized pigs and an equal number of age-matched unimmunized control pigs were all challenged by intravenous injection of purified SLT-IIv (6 ng/kg body weight). The six passively immunized pigs acquired neutralizing SLT-IIv antibody titers of 1280 or 2560 and the 18 actively immunized pigs mounted a serum immune response with toxin neutralizing titers ranging from 128 to 10,240. All 24 immunized pigs survived the challenge but nine pigs with the lowest titers showed mild clinical signs of edema disease. All 24 control pigs died within 30 hours of challenge.
采用中和抗体的被动转移以及用纯化的志贺样毒素II变体（SLT-IIv，水肿病毒素）类毒素进行主动免疫，来保护猪免受SLT-IIv攻击。6头6周龄仔猪通过腹腔注射来自抗纯化SLT-IIv猪抗血清的免疫球蛋白制剂进行被动免疫。6头6周龄仔猪和12头2周龄仔猪通过间隔2周进行两次肌肉注射25微克SLT-IIv类毒素进行主动免疫。24头免疫仔猪和相同数量的年龄匹配未免疫对照仔猪均通过静脉注射纯化的SLT-IIv（6纳克/千克体重）进行攻击。6头被动免疫仔猪获得了1280或2560的中和SLT-IIv抗体效价，18头主动免疫仔猪产生了血清免疫反应，毒素中和效价范围为128至10240。所有24头免疫仔猪在攻击中存活，但效价最低的9头仔猪出现了水肿病的轻微临床症状。所有24头对照仔猪在攻击后30小时内死亡。
