Shimomura T, Izawa C, Matsui T
Funai Electric Co., Ltd., 1-1 Minami-watarida-chou, Kawasaki-ku, Kawasaki-shi, Kanagawa 210-0855, Japan.
Rev Sci Instrum. 2008 Mar;79(3):035101. doi: 10.1063/1.2885609.
We present a compact optical system using a commercially available DVD pickup head for microarray scanning. Our instrument successfully provides a low-cost, compact, and simple microarray scanning optical system in comparison to conventional ones due to the use of small-sized optical components and the implementation of a simple autofocusing system using an embedded voice coil motor. The performance of this system was validated by using a microarray slide with spots of fluorescent dyes. It was confirmed that our optical head performed satisfactorily and was suitable for practical use in microarray scanners. This result provides evidence of the superiority of our microarray scanning optical system over conventional ones because of its space-saving properties and cost effectiveness.
我们展示了一种使用市售DVD拾音头的紧凑型光学系统用于微阵列扫描。与传统系统相比,我们的仪器成功提供了一种低成本、紧凑且简单的微阵列扫描光学系统,这得益于使用了小型光学元件以及采用嵌入式音圈电机实现了简单的自动聚焦系统。通过使用带有荧光染料斑点的微阵列载玻片对该系统的性能进行了验证。结果证实我们的光学头性能令人满意,适用于微阵列扫描仪的实际应用。这一结果证明了我们的微阵列扫描光学系统相较于传统系统具有优越性,因其具有节省空间的特性和成本效益。