Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Micro- and Nanotechnology , Technical University of Denmark , Lyngby 2800 , Denmark.
ACS Sens. 2018 Jul 27;3(7):1222-1232. doi: 10.1021/acssensors.8b00340. Epub 2018 Jul 18.
The optical pickup unit (OPU) within a CD/DVD/Blu-ray drive integrates 780, 650, and 405 nm wavelength lasers, diffraction-limited optics, a high-bandwidth optoelectronic transducer up to 400 MHz, and a nanoresolution x-, z-axis, and tilt actuator in a compact size. In addition, the OPU is a remarkable piece of engineering and could enable different scientific applications such as sub-angstrom displacement sensing, micro- and nanoimaging, and nanolithography. Although off-the-shelf OPUs can be easily obtained, manufacturers protect their datasheets under nondisclosure agreements to impede their availability to the public. Thus, OPUs are black boxes that few people can use for research, and only experienced researchers can access all their functions. This review details the OPU mechanism and components. In addition, we explain how to utilize three commercially available triple-wavelength OPUs from scratch and optimize sensing quality. Then, we discuss scientific research using OPUs, from standard optical drive-based turnkey-biomarker array reading and OPU direct bioapplications (cytometry, optical tweezing, bioimaging) to modified OPU-based biosensing (DNA chip fluorescence scanning, biomolecular diagnostics). We conclude by presenting future trends on optical storage devices and potential applications. Hacking low-cost and high-performance OPUs may spread micro- and nanoscale biosensing research from research laboratories to citizen scientists around the globe.
CD/DVD/Blu-ray 驱动器中的光学拾光单元 (OPU) 集成了 780、650 和 405nm 波长的激光、衍射极限光学器件、高达 400MHz 的高带宽光电换能器以及纳米分辨率的 x、z 轴和倾斜执行器，尺寸小巧。此外，OPU 是一项杰出的工程成就，可以实现不同的科学应用，如亚埃级别的位移传感、微纳成像和纳米光刻。尽管可以轻松获得现成的 OPU，但制造商根据保密协议保护其数据表，以阻止其向公众公开。因此，OPU 是一个很少有人可以用于研究的黑盒子，只有经验丰富的研究人员才能访问其所有功能。本文详细介绍了 OPU 的工作原理和组成部分。此外，我们还解释了如何从零开始利用三个市售的三波长 OPU，并优化传感质量。然后，我们讨论了使用 OPU 的科学研究，从基于标准光学驱动器的即用型生物标记物阵列读取和 OPU 直接生物应用（细胞计数、光镊、生物成像）到基于改良 OPU 的生物传感（DNA 芯片荧光扫描、生物分子诊断）。最后，我们展示了光学存储设备的未来趋势和潜在应用。黑客攻击低成本、高性能的 OPU 可能会将微纳尺度生物传感研究从研究实验室传播到全球的公民科学家手中。
