Ragozzino M M, Kuo A, DeGregori J, Kohl N, Ruley H E
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Environ Health Perspect. 1991 Jun;93:97-103. doi: 10.1289/ehp.919397.
Gene transfer experiments have defined limitations with regard to the ability of individual oncogenes to transform cultured cells to a tumorigenic state. The stable transformation of REF52 cells by either the ras or sis oncogenes requires the continuous expression of a second collaborating oncogene, such as adenovirus-5 E1A or SV40 large T-antigen. Our studies suggest that the function of the nuclear collaborators is to antagonize dominant growth controls which limit the ability of REF52 cells to proliferate in response to mitogenic stimuli.
基因转移实验已经明确了个别癌基因将培养细胞转化为致瘤状态的能力存在局限性。ras或sis癌基因对REF52细胞的稳定转化需要第二种协同癌基因的持续表达,如腺病毒5型E1A或SV40大T抗原。我们的研究表明,核内协同基因的功能是对抗显性生长控制,这种控制限制了REF52细胞响应有丝分裂刺激而增殖的能力。