Cook J L, May D L, Wilson B A, Walker T A
Robert W. Lisle Research Laboratory in Immunology and Tumor Cell Biology, Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
J Virol. 1989 Aug;63(8):3408-15. doi: 10.1128/JVI.63.8.3408-3415.1989.
The E1A oncogene of adenovirus serotypes 2 and 5 induces susceptibility to the cytolytic effects of natural killer lymphocytes and activated macrophages when expressed in infected and transformed mammalian cells (cytolysis-susceptible phenotype). E1A and the oncogenes v-myc, long-terminal-repeat-promoted c-myc, and activated c-ras share the ability to immortalize transfected low-passage rodent cells. The cytolytic phenotypes of well-characterized rodent cell lines immortalized by these three oncogenes were defined. In contrast to target cells expressing the intact E1A gene, myc- and ras-expressing, immortalized primary transfectants were resistant to lysis by both types of killer cell populations. The same patterns of susceptibility (E1A) and resistance (myc and ras) to cytolysis were observed in oncogene-transfected continuous rat (REF52) and mouse (NIH 3T3) cell lines, indicating that differences in the cytolytic phenotypes associated with expression of these oncogenes are not due to cell selection during immortalization. The results suggest that the E1A oncogene may possess a functional domain that is different from those of other oncogenes, such as myc and ras, and that the activity linked to this postulated domain is dissociable from the process of immortalization.
腺病毒2型和5型的E1A癌基因在受感染和转化的哺乳动物细胞中表达时（细胞溶解敏感表型），会诱导细胞对自然杀伤淋巴细胞和活化巨噬细胞的细胞溶解作用产生敏感性。E1A与癌基因v-myc、长末端重复序列促进的c-myc以及活化的c-ras都具有使转染的低代啮齿动物细胞永生化的能力。确定了由这三种癌基因永生化的特征明确的啮齿动物细胞系的细胞溶解表型。与表达完整E1A基因的靶细胞不同，表达myc和ras的永生化原代转染细胞对这两种杀伤细胞群体的裂解具有抗性。在癌基因转染的连续大鼠（REF52）和小鼠（NIH 3T3）细胞系中也观察到了对细胞溶解的相同敏感性（E1A）和抗性（myc和ras）模式，这表明与这些癌基因表达相关的细胞溶解表型差异并非由于永生化过程中的细胞选择所致。结果表明，E1A癌基因可能具有一个与其他癌基因（如myc和ras）不同的功能域，并且与这个假定域相关的活性与永生化过程是可分离的。
