重组表达的带（His）6标签的花生过敏原（rAra h 1）的生物活性不受内毒素去除的影响。
The biological activity of a recombinantly expressed (His)(6)-tagged peanut allergen (rAra h 1) is unaffected by endotoxin removal.
作者信息
Jensen Louise Bjerremann, Torp Anna Maria, Andersen Sven Bode, Skov Per Stahl, Poulsen Lars K, Knol Edward F, van Hoffen Els
机构信息
Department of Agricultural Sciences, Faculty of Life Sciences, University of Copenhagen, Denmark.
出版信息
J Immunol Methods. 2008 Jun 1;335(1-2):116-20. doi: 10.1016/j.jim.2008.02.012. Epub 2008 Mar 11.
The application of recombinant (His)(6)-tagged proteins in cell culture assays is associated with problems due to lipopolysaccharide (LPS) contamination. LPS stimulates cells of the immune system, thereby masking antigen-specific activation of T cells. Due to the affinity of LPS for histidine it is associated with difficulties to remove LPS from recombinant (His)(6)-tagged proteins. Here we describe that the Triton X-114 phase separation method can be used to remove LPS from (His)(6)-tagged proteins and that the recombinant proteins retain their biological activity.
由于脂多糖（LPS）污染，重组（His）6标记蛋白在细胞培养分析中的应用存在问题。LPS刺激免疫系统细胞，从而掩盖T细胞的抗原特异性激活。由于LPS对组氨酸的亲和力，从重组（His）6标记蛋白中去除LPS存在困难。在此我们描述了Triton X-114相分离方法可用于从（His）6标记蛋白中去除LPS，且重组蛋白保留其生物学活性。
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