Nakajima Hiroyuki, Yonemura Shigenobu, Murata Masayuki, Nakamura Nobuhiro, Piwnica-Worms Helen, Nishida Eisuke
Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
J Cell Biol. 2008 Apr 7;181(1):89-103. doi: 10.1083/jcb.200708176. Epub 2008 Mar 31.
Myt1 was originally identified as an inhibitory kinase for Cdc2 (Cdk1), the master engine of mitosis, and has been thought to function, together with Wee1, as a negative regulator of mitotic entry. In this study, we report an unexpected finding that Myt1 is essential for Golgi and endoplasmic reticulum (ER) assembly during telophase in mammalian cells. Our analyses reveal that both cyclin B1 and cyclin B2 serve as targets of Myt1 for proper Golgi and ER assembly to occur. Thus, our results show that Myt1-mediated suppression of Cdc2 activity is not indispensable for the regulation of a broad range of mitotic events but is specifically required for the control of intracellular membrane dynamics during mitosis.
Myt1最初被鉴定为有丝分裂的主要驱动因子Cdc2（Cdk1）的抑制性激酶，并且一直被认为与Wee1一起作为有丝分裂进入的负调节因子发挥作用。在本研究中，我们报告了一个意外发现，即Myt1对于哺乳动物细胞末期高尔基体和内质网（ER）的组装至关重要。我们的分析表明，细胞周期蛋白B1和细胞周期蛋白B2都是Myt1的靶标，以确保高尔基体和内质网的正常组装。因此，我们的结果表明，Myt1介导的Cdc2活性抑制对于广泛的有丝分裂事件的调节并非不可或缺，但对于有丝分裂期间细胞内膜动力学的控制是特别必需的。
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