Kulish Iu S, Kashkin K P
Radiats Biol Radioecol. 2007 Nov-Dec;47(6):707-16.
Rats were treated with a single intravenous injection of thorotrast (thorium dioxide)--the source of alpha-rays. Dynamic investigation of urine protens of rats by methods of electrophoresis and immunoelectrophoresis was carried out during 22 months after thorotrast injection. Already the month after drug injection the selectivity of tubular reabsorbtion was disturbed. Three months after thorotrast injection the content of urinal proteins of tissue (in particular renal) origin was decreased. Finally the selectivity of renal filtration of proteins was damaged 4-6 months after thorotrast introduction. Serum proteins which were absent in normal urine (for example transferrin and lipoproteins) appeared in urine of affected rats. The urine proteins of serum origin were less degraded than those in normal urine. The alterations of glomerular filtration was increased up to 20-22 months when the spectrum of urine proteins became similar to the spectrum of serum proteins. The death of treated rats was occurred in this period. Thus the monitoring of urine proteins of rats treated with alpha-ray producing preparation throtrast allows to register the successive alterations of reabsorbtion, excretion and filtration functions of kidney.
给大鼠单次静脉注射钍造影剂（二氧化钍）——α射线源。在注射钍造影剂后的22个月内，采用电泳和免疫电泳方法对大鼠尿蛋白进行动态研究。在注射药物后的第一个月，肾小管重吸收的选择性就受到了干扰。注射钍造影剂三个月后，组织（特别是肾脏）来源的尿蛋白含量降低。最终，在引入钍造影剂4 - 6个月后，肾脏对蛋白质的滤过选择性受到损害。正常尿液中不存在的血清蛋白（如转铁蛋白和脂蛋白）出现在患病大鼠的尿液中。血清来源的尿蛋白比正常尿液中的降解程度更低。肾小球滤过的改变在20 - 22个月时加剧，此时尿蛋白谱与血清蛋白谱相似。在此期间，接受治疗的大鼠死亡。因此，对用产生α射线的制剂钍造影剂处理的大鼠的尿蛋白进行监测，可以记录肾脏重吸收、排泄和滤过功能的连续变化。
