Hoefig Kai P, Thorns Christoph, Roehle Anja, Kaehler Christian, Wesche Kai O, Repsilber Dirk, Branke Biggi, Thiere Marlen, Feller Alfred C, Merz Hartmut
Institute for Pathology, UKSH Campus Luebeck, Luebeck, Germany.
Anticancer Res. 2008 Jan-Feb;28(1A):119-23.
MicroRNAs (miRNAs) are approximately 22 nucleotide long, non-coding RNAs that regulate gene expression by binding to the 3'-untranslated region of target mRNAs and also a variety of cellular processes. It has recently been established that dysregulation of miRNA expression can be detected in the majority of human cancers. A variety of high-throughput screening methods has been developed to identify dysregulated miRNA species in tumours. For retrospective clinical studies formalin-fixed, paraffin-embedded (FFPE) tissue is the most widely used material.
The miRNA expression profiles of freshly frozen (CRYO) and FFPE tissues of seven tonsil and four liver samples were compared, using a qPCR-based assay, profiling 157 miRNA species.
The significance of miRNA-profiles was barely influenced by FFPE treatment in both tissues and the variance induced by FFPE treatment was much smaller than the variance caused by biologically based differential expression.
FFPE material is well suited for miRNA profiling.
微小RNA（miRNA）是长度约为22个核苷酸的非编码RNA，通过与靶mRNA的3'非翻译区结合来调控基因表达，同时也参与多种细胞过程。最近已证实，在大多数人类癌症中均可检测到miRNA表达失调。已开发出多种高通量筛选方法来鉴定肿瘤中失调的miRNA种类。对于回顾性临床研究，福尔马林固定、石蜡包埋（FFPE）组织是使用最广泛的材料。
使用基于qPCR的分析方法，对7个扁桃体和4个肝脏样本的新鲜冷冻（CRYO）组织和FFPE组织的miRNA表达谱进行比较，并对157种miRNA进行分析。
在两种组织中，FFPE处理对miRNA谱的影响微乎其微，且FFPE处理引起的差异远小于生物学差异表达所导致的差异。
FFPE材料非常适合用于miRNA分析。
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