Paternolli Cristina, Neebe Martin, Stura Enrico, Barbieri Francesca, Ghisellini Paola, Hampp Norbert, Nicolini Claudio
Fondazione Elba, Piazza SS, Apostoli 66, 00100 Rome, Italy.
J Biomed Mater Res A. 2009 Mar 15;88(4):947-51. doi: 10.1002/jbm.a.31925.
In this work, a new biomaterial resulting from the isolation of octopus rhodopsin (OR) starting from octopus photoreceptor membranes is presented. Mass spectroscopic characterization was employed in order to verify the presence of rhodopsin in the extract. Photoreversibility and photochromic properties were investigated using spectrophotometric measurements and pulsed light. Thin films of OR were realized using the gel-matrix entrapment method in polyvinyl alcohol solution. The results indicate that the photoreversibility and the photostability of the OR in gel-matrices are maintained. Several measurements were performed to test the stability of the resulting biomaterial in time and at room temperature. Preliminary tests demonstrate that the photoreversibility and the photostability are still found after few days from the biomaterial preparation and after the exposure for several hours at room temperature.
在这项工作中，展示了一种从章鱼光感受器膜中分离出章鱼视紫红质（OR）而得到的新型生物材料。采用质谱表征来验证提取物中视紫红质的存在。使用分光光度测量和脉冲光研究了光可逆性和光致变色特性。通过凝胶基质包埋法在聚乙烯醇溶液中制备了OR薄膜。结果表明，凝胶基质中OR的光可逆性和光稳定性得以保持。进行了多项测量以测试所得生物材料在时间和室温下的稳定性。初步测试表明，在生物材料制备几天后以及在室温下暴露数小时后，仍能观察到光可逆性和光稳定性。
