Rempel Mary Ann, Wang Yinsheng, Armstrong Jeff, Schlenk Daniel
Environmental Toxicology Program, University of California Riverside, Riverside, CA, USA.
Mar Environ Res. 2008 Jul;66(1):111-2. doi: 10.1016/j.marenvres.2008.02.039. Epub 2008 Feb 26.
Male and female hornyhead turbot (Pleuronichthys verticalis) were exposed to four concentrations (0, 0.75, 14.7 and 46.5 ng/g dry weight) of E2-amended sediment for 7 days. Sediment-derived E2 was bioavailable to the flatfish, though the route of uptake was unclear. A concentration of 46.5 ng/g E2 in sediment led to a significant increase in vitellogenin concentrations in the plasma in both sexes after 7 days of exposure. Though plasma E2 concentrations increased significantly in males at sediment E2 concentrations of 0.75 ng/g dry weight and above, a dose-dependent increase was not observed. There was also no correlation between sediment E2 concentrations, plasma E2 concentrations, and oxidative DNA damage in male gonads. The results suggest that the DNA damage previously seen in the gonads of feral hornyhead turbot at a sewage outfall is likely not caused by acute exposure to exogenous E2 from sediments.
将雄性和雌性角头鲽（Pleuronichthys verticalis）暴露于四种浓度（0、0.75、14.7和46.5纳克/克干重）添加了雌二醇（E2）的沉积物中7天。沉积物中的E2对这种比目鱼具有生物可利用性，尽管吸收途径尚不清楚。暴露7天后，沉积物中46.5纳克/克的E2浓度导致两性血浆中卵黄蛋白原浓度显著增加。尽管在沉积物E2浓度为0.75纳克/克干重及以上时，雄性血浆E2浓度显著增加，但未观察到剂量依赖性增加。雄性性腺中沉积物E2浓度、血浆E2浓度与氧化性DNA损伤之间也没有相关性。结果表明，之前在污水排放口野生角头鲽性腺中观察到的DNA损伤可能不是由沉积物中外源E2的急性暴露引起的。
