Lee Gowoun, Joo Hongil, Kim Jungbae, Lee Jung-Heon
Department of Chemical Engineering, Chosun University, Kwangju 501-759, Korea.
J Microbiol Biotechnol. 2008 Mar;18(3):465-71.
Highly active, stable, and magnetically separable immobilized enzymes were developed using carboxymethyl cellulose (CMC) and diethylaminoethyl cellulose DEAE-C; hereafter designated "DEAE" as supporting materials. Iron oxide nanoparticles penetrated the micropores of the supporting materials, rendering them magnetically separable. Lipase (LP) was immobilized on the surface of the supporting materials by using cross-linked enzyme aggregation (CLEA) by glutaraldehyde. The activity of enzyme aggregates coated on DEAE was approximately 2 times higher than that of enzyme aggregates coated on CMC. This is explained by the fact that enzyme aggregates with amine residues are more efficient than those with carboxyl residues. After a 96-h enantioselective ibuprofen esterification reaction, 6% ibuprofen propyl ester was produced from the racemic mixture of ibuprofen by using DEAE-LP, and 2.8% using CMC-LP.
利用羧甲基纤维素（CMC）和二乙氨基乙基纤维素DEAE-C（以下简称“DEAE”）作为支撑材料，制备了高活性、稳定且具有磁分离性能的固定化酶。氧化铁纳米颗粒渗透到支撑材料的微孔中，使其具有磁分离性能。通过戊二醛交联酶聚合法（CLEA）将脂肪酶（LP）固定在支撑材料表面。涂覆在DEAE上的酶聚集体的活性约为涂覆在CMC上的酶聚集体活性的2倍。这可以通过带有胺残基的酶聚集体比带有羧基残基的酶聚集体更有效的事实来解释。在进行96小时的布洛芬对映选择性酯化反应后，使用DEAE-LP从布洛芬的外消旋混合物中产生了6%的布洛芬丙酯，而使用CMC-LP产生了2.8%的布洛芬丙酯。
