固定化假丝酵母脂肪酶拆分消旋布洛芬。
Immobilization of Candida rugosa lipase for resolution of racimic ibuprofen.
机构信息
Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
出版信息
Daru. 2021 Jun;29(1):117-123. doi: 10.1007/s40199-021-00388-7. Epub 2021 Feb 2.
AIM
Due to lipases' regio-selectivity and ability to catalyze different reactions such as hydrolysis, esterification, and transesterification, the enzyme is attractive in biotransformation technology. Besides, another technology, namely enzyme immobilization, has attracted scientists/technologists' attention to employ immobilized lipase in such a field. Thus lipase of Candida rugosa was immobilized onto silica nanoparticles through adsorption. Furthermore, the immobilized biocatalyst was characterized and used to esterify ibuprofen enantioselectively.
METHODS
To characterize immobilized lipase onto silica nanoparticles scanning electron microscopy (SEM) and dynamic light scattering (DLS) were used.
RESULTS
The catalytic properties of both immobilized and free lipases such as optima pH and temperature were not different. According to the results, the immobilized lipase on silica nanoparticles showed 45% and 96% conversion (C) and enantioselectivity (ee), respectively. In comparison to free lipase, the immobilized enzyme came with better catalytic activity.
CONCLUSION
Silica nanoparticles as one of the most promising materials for the immobilization of lipase in enantioselective esterification of ibuprofen, were introduced in this work.
目的
由于脂肪酶具有区域选择性和催化不同反应的能力，如水解、酯化和酯交换反应，因此该酶在生物转化技术中具有吸引力。此外，另一种技术，即酶固定化，引起了科学家/技术人员的关注，他们希望在该领域使用固定化脂肪酶。因此，通过吸附将假丝酵母脂肪酶固定到硅胶纳米颗粒上。此外，对固定化生物催化剂进行了表征，并用于对布洛芬进行对映选择性酯化。
方法
使用扫描电子显微镜（SEM）和动态光散射（DLS）对固定在硅胶纳米颗粒上的脂肪酶进行了表征。
结果
固定化和游离脂肪酶的催化特性，如最适 pH 和温度，没有差异。结果表明，固定在硅胶纳米颗粒上的脂肪酶的转化率（C）和对映选择性（ee）分别为 45%和 96%。与游离脂肪酶相比，固定化酶具有更好的催化活性。
结论
本工作介绍了硅胶纳米颗粒作为脂肪酶在布洛芬对映选择性酯化中固定化的最有前途的材料之一。
Zotero 插件