Aguado M, Martinez-Urbistondo D, Solomon A, Martinez J A
Department of Physiology and Nutrition, University of Navarra, Pamplona, Spain.
Methods Find Exp Clin Pharmacol. 2008 Jan-Feb;30(1):7-11. doi: 10.1358/mf.2008.30.1.1147772.
Lipid turnover and deposition is under the control of nutritional, developmental, metabolic and neuroendocrine influences. The aim of this investigation was to study whether there is a gender-dependent difference in the effect of leptin in lipolytic activity of freshly isolated human fat cells. The lipolytic rate was assessed through the glycerol release after incubation with leptin at different concentrations ranging from 10(-6) to 10(-12) M in isolated adipocytes obtained from male and female omental adipose tissue. The relative ED(50) concentration of leptin was found to be 4.42 x 10(-10) M in adipocytes from men and 3.08 x 10(-12) M in fat cells from women. The presence of leptin at concentrations of 10(-12) to 10(-6) M in the incubation medium of isolated fat cells significantly increased glycerol release when compared with basal levels, being always higher (8-15%) in female adipocytes when compared with male specimens for all tested concentrations when expressed on a fat content relative basis. This study demonstrates gender differences regarding the lipolytic actions of leptin, which may help the pharmacological application of leptin-based therapies.
脂质周转和沉积受营养、发育、代谢及神经内分泌影响的调控。本研究的目的是探讨瘦素对新鲜分离的人脂肪细胞脂解活性的影响是否存在性别差异。通过在从男性和女性网膜脂肪组织获取的分离脂肪细胞中，用不同浓度（范围从10⁻⁶到10⁻¹²M）的瘦素孵育后测定甘油释放来评估脂解速率。发现男性脂肪细胞中瘦素的相对半数有效浓度（ED₅₀）为4.42×10⁻¹⁰M，女性脂肪细胞中为3.08×10⁻¹²M。与基础水平相比，在分离脂肪细胞的孵育培养基中存在浓度为10⁻¹²到10⁻⁶M的瘦素时，甘油释放显著增加，在所有测试浓度下，以脂肪含量相对为基础表示时，女性脂肪细胞中的甘油释放始终比男性标本高（8 - 15%）。本研究证明了瘦素脂解作用存在性别差异，这可能有助于基于瘦素疗法的药理学应用。
