Cammack J, Tessel R, Michaelis E, Adams R N
Department of Pharmacology and Toxicology, University of Kansas, Lawrence 66045.
Eur J Pharmacol. 1991 Aug 16;201(1):111-3. doi: 10.1016/0014-2999(91)90330-s.
Antioxidants such as ascorbic acid (AA) and dithiothreitol (DTT), can prevent 3,4-dihydroxyphenylacetic acid (DOPAC) inhibition of [3H]spiperone binding to neuronal dopamine D2 receptors. The DOPAC quinone produced from auto-oxidation is believed to be responsible for the irreversible inhibition noted. Quinone conjugation to proteins occurs readily in vivo, and thus, auto-oxidation of DOPAC and other endogenous catechol containing compounds could be an important component of protein modification during on-going neuronal physiology.
抗氧化剂如抗坏血酸（AA）和二硫苏糖醇（DTT），可以防止3,4 - 二羟基苯乙酸（DOPAC）对[3H] - 螺哌隆与神经元多巴胺D2受体结合的抑制作用。据信，由自动氧化产生的DOPAC醌是造成上述不可逆抑制的原因。醌与蛋白质的共轭反应在体内很容易发生，因此，DOPAC和其他含内源性儿茶酚的化合物的自动氧化可能是正在进行的神经元生理过程中蛋白质修饰的一个重要组成部分。
