Long Shuang-lian, Liu Fen, Wang Ting-hua, Xu Xin-yun, Guang Yu-guang, Wang Te-wei, Ke Qing, Yuan Yuan
Institute of Neuroscience, Kunming Medical College, Kunming 650031, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2005 Jan;36(1):35-8.
This study was conducted to detect the expression levels of NT-4 in the spared dorsal root ganglia (DRG, L6) on the operated side and un-operated side, in the spinal lamina II (L3, L5, L6) and Clarke's nucleus (L3) of the normal adult cats and the cats submitted to partial dorsal rhizotomy. The aim was to illuminate the temporospatial changes of NT-4 expression and its possible relation to spinal cord plasticity.
Fifteen male adult cats were divided into three groups. The cats of two groups were submitted to unilateral partial dorsal root rhizotomy (L1-L5, L7-S2 DRG were sectioned, but L6 was spared) and animals were sacrificed at survived 7 days and 14 days after operation. The sections were stained by the immunohistochemistry ABC method under the same condition, using NT-4 (1:200, Santa Cruz) antibody. The distribution and the number of NT-4 positive neurons in bilateral spared DRG (L6) in the operated/un-operated side, spinal lamina II (L3, L5, L6) and Clarke's nucleus (L3) of each animal were observed and counted. All data were analyzed by one-way ANOVA, SNK-q test and paired-t test.
(1) Seven days after partial dorsal root rhizotomy, the number of NT-4 positive neurons in spared DRG in the operated side was significantly less than that of normal group (P<0.05), but significantly more than that of L6 DRG in the unoperated side (P<0.05); the number of NT-4 positive neurons of L6 DRG on the 14th day after partial dorsal root rhizotomy was also less than that of normal group, 7-day group, and the un-operated side (P<0.05). (2) There was no difference in respect to the number of NT-4 positive neuron in L3, L5, L6 spinal lamina II and L3 Clarke's nucleus in the operated side between the normal group, 7-day operation group and 14-day operation group (P>0.05).
Our results suggest that partial dorsal root rhizotomy can mainly lead to the change of NT-4 expression in the spared root ganglion, and NT-4 in the unoperated side DRG are involved in spinal cord plasticity.
本研究旨在检测正常成年猫及接受部分背根切断术的猫的手术侧和非手术侧备用背根神经节（DRG，L6）、脊髓板层II（L3、L5、L6）和克拉克核（L3）中NT-4的表达水平。目的是阐明NT-4表达的时空变化及其与脊髓可塑性的可能关系。
15只成年雄性猫分为三组。两组猫接受单侧部分背根切断术（切断L1-L5、L7-S2 DRG，但保留L6），并在术后存活7天和14天时处死动物。在相同条件下，使用NT-4（1:200，Santa Cruz）抗体通过免疫组织化学ABC法对切片进行染色。观察并计数每只动物手术侧/非手术侧双侧备用DRG（L6）、脊髓板层II（L3、L5、L6）和克拉克核（L3）中NT-4阳性神经元的分布和数量。所有数据采用单因素方差分析、SNK-q检验和配对t检验进行分析。
（1）部分背根切断术后7天，手术侧备用DRG中NT-4阳性神经元数量明显少于正常组（P<0.05），但明显多于非手术侧L6 DRG（P<0.05）；部分背根切断术后14天，L6 DRG中NT-4阳性神经元数量也少于正常组、7天组和非手术侧（P<0.05）。（2）正常组、7天手术组和14天手术组手术侧L3、L5、L6脊髓板层II和L3克拉克核中NT-4阳性神经元数量无差异（P>0.05）。
我们的结果表明，部分背根切断术主要导致备用神经根节中NT-4表达的变化，非手术侧DRG中的NT-4参与脊髓可塑性。
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