Biterova Ekaterina I, Svärd Maria, Possner Dominik D D, Guy Jodie E
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Tomtebodavägen 6, S171-77 Stockholm, Sweden.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 May 1;69(Pt 5):544-6. doi: 10.1107/S1744309113008063. Epub 2013 Apr 30.
The membrane protein Erv41p is a major component of COPII-coated vesicles and is thought to play a role in the early secretory pathway in eukaryotic cells. In this study, the full lumenal domain of Erv41p from Saccharomyces cerevisiae (ScErv41p_LD) was recombinantly expressed in Sf9 insect cells and purified by nickel-affinity, ion-exchange and size-exclusion chromatography. ScErv41p_LD crystals were obtained using the sitting-drop vapour-diffusion method and native X-ray diffraction data were collected to 2.0 Å resolution. The crystals belonged to space group P21, with unit-cell parameters a = 49.8, b = 76.9, c = 65.1 Å, α = γ = 90.0, β = 104.8°.
膜蛋白Erv41p是COPII被膜小泡的主要成分，被认为在真核细胞的早期分泌途径中发挥作用。在本研究中，酿酒酵母的Erv41p的完整腔结构域（ScErv41p_LD）在Sf9昆虫细胞中重组表达，并通过镍亲和、离子交换和尺寸排阻色谱法进行纯化。使用坐滴气相扩散法获得了ScErv41p_LD晶体，并收集了分辨率为2.0 Å的天然X射线衍射数据。晶体属于空间群P21，晶胞参数为a = 49.8、b = 76.9、c = 65.1 Å，α = γ = 90.0，β = 104.8°。
