对过表达磷酸烯醇丙酮酸羧激酶的大肠杆菌进行的生理学研究。
A physiology study of Escherichia coli overexpressing phosphoenolpyruvate carboxykinase.
作者信息
Kwon Yeong-Deok, Lee Sang Yup, Kim Pil
机构信息
Department of Life Science, Catholic University of Korea, Bucheon, Gyunggi 420-743, Korea.
出版信息
Biosci Biotechnol Biochem. 2008 Apr;72(4):1138-41. doi: 10.1271/bbb.70831. Epub 2008 Apr 7.
To determine whether intracellular ATP levels can be affected, Escherichia coli overexpressing phosphoenolpyruvate carboxykinase (pck) or phosphoenolpyruvate carboxylase (ppc) were grown in glucose minimal medium. The Pck-overexpressing cells showed approximately twice the intracellular ATP concentration, with 22% slower growth than the Ppc-overexpressing strain. This unexpected result of higher ATP coupled with slower growth is discussed based on transcriptome analysis.
为了确定细胞内ATP水平是否会受到影响，在葡萄糖基本培养基中培养过表达磷酸烯醇式丙酮酸羧激酶（Pck）或磷酸烯醇式丙酮酸羧化酶（Ppc）的大肠杆菌。过表达Pck的细胞显示细胞内ATP浓度约为原来的两倍，但其生长速度比过表达Ppc的菌株慢22%。基于转录组分析讨论了这种ATP水平升高但生长速度减慢的意外结果。
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