产琥珀酸放线杆菌磷酸烯醇式丙酮酸羧激酶过表达对大肠杆菌琥珀酸生产的影响
Effect of overexpression of Actinobacillus succinogenes phosphoenolpyruvate carboxykinase on succinate production in Escherichia coli.
作者信息
Kim Pil, Laivenieks Maris, Vieille Claire, Zeikus J Gregory
机构信息
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA.
出版信息
Appl Environ Microbiol. 2004 Feb;70(2):1238-41. doi: 10.1128/AEM.70.2.1238-1241.2004.
Abstract
Succinate fermentation was investigated in Escherichia coli strains overexpressing Actinobacillus succinogenes phosphoenolpyruvate carboxykinase (PEPCK). In E. coli K-12, PEPCK overexpression had no effect on succinate fermentation. In contrast, in the phosphoenolpyruvate carboxylase mutant E. coli strain K-12 ppc::kan, PEPCK overexpression increased succinate production 6.5-fold.
摘要
在过表达琥珀酸放线杆菌磷酸烯醇式丙酮酸羧激酶（PEPCK）的大肠杆菌菌株中研究了琥珀酸发酵。在大肠杆菌K-12中，过表达PEPCK对琥珀酸发酵没有影响。相反，在磷酸烯醇式丙酮酸羧化酶突变型大肠杆菌菌株K-12 ppc::kan中，过表达PEPCK使琥珀酸产量提高了6.5倍。
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