Liu Yun-Guo, Liu Ling-Xiao, Wang Lei, Gao Ai-Ying
Division of Life Science and Technology, Ocean University of China, Qingdao, 266003, China.
Cryo Letters. 2008 Jan-Feb;29(1):7-14.
In vitro-grown apple shoot tips were successfully cryopreserved by vitrification, with an average survival and shoot formation of 80 percent and 76 percent respectively. Surviving shoots showed the same rate and regrowth patterns as those of non-treated controls. No significant differences (P < 0.05) were observed in morphological characteristics, including shoot length, leaf shape, leaf width/length ratio and root length, between the control and cryopreserved shoots. No different microsatellite alleles and ISSR fragments were detected between control and cryopreserved shoots using twelve pairs of microsatellites and eleven ISSR primers. These results show that cryopreservation using vitrification is a practical method for the long-term storage of apple germplasm.
通过玻璃化法成功对离体培养的苹果茎尖进行了超低温保存，平均存活率和芽形成率分别为80%和76%。存活的芽与未处理的对照芽表现出相同的生长速率和再生长模式。对照芽和超低温保存芽在形态特征上，包括茎长、叶形、叶宽/长比和根长，均未观察到显著差异（P < 0.05）。使用12对微卫星引物和11条ISSR引物，在对照芽和超低温保存芽之间未检测到不同的微卫星等位基因和ISSR片段。这些结果表明，玻璃化超低温保存是苹果种质长期保存的一种实用方法。
