Liu L
Institute of Basic Medical Sciences, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1991 Oct;13(5):363-6.
Monoclonal antibodies (Lc86a-C5, Lc86a-H8) directed against human lung adenocarcinoma cell line LTEP-a-2 and normal BALB/c IgG were labelled with iodine-131 by chloramine T. The 131I-McAbs and 131I-IgG were respectively injected into the peritoneal cavities of nude mice bearing transplanted human lung adenocarcinoma cell line LTEP-a-2. After 72 h, the tumor tissue in nude mice injected with 131I-McAbs was distinguishable from normal tissues as a very clear image obtained during gamma scintigraphy. No difference was found between tumor and normal tissues in the nude mice injected with 131I-IgG. The tumor: blood ratio was 3.1:1 in nude mice injected with 131I-McAb(H8) and 0.9:1 in nude mice injected with 131I-IgG respectively. This indicates that the tumor tissue image was the result of specific binding of the 131I-McAbs, which have high specificity and affinity both in vitro and in vivo, to tumor cells, and these monoclonal antibodies may serve as potential agents in tumor diagnosis and treatment.
用氯胺T法将抗人肺腺癌细胞系LTEP-a-2的单克隆抗体（Lc86a-C5、Lc86a-H8）及正常BALB/c IgG标记上碘-131。将131I-单克隆抗体和131I-IgG分别注入荷人肺腺癌细胞系LTEP-a-2移植瘤的裸鼠腹腔内。72小时后，注射131I-单克隆抗体的裸鼠肿瘤组织在γ闪烁显像时呈现为非常清晰的影像，可与正常组织区分开来。注射131I-IgG的裸鼠肿瘤组织与正常组织无差异。注射131I-单克隆抗体（H8）的裸鼠肿瘤与血液的比值为3.1:1，注射131I-IgG的裸鼠该比值为0.9:1。这表明肿瘤组织影像系131I-单克隆抗体在体内外均具有高特异性和亲和力，与肿瘤细胞特异性结合的结果，这些单克隆抗体可能成为肿瘤诊断和治疗的潜在药物。
