Liu Y, Wu M C, Qian G X
Institute of Hepatobiliary Surgery, Second Military Medical University, Shanghai.
Zhonghua Wai Ke Za Zhi. 1994 Nov;32(11):650-3.
Anti-human AFP variant monoclonal antibody (AFP-R-LCA McAb) was labeled by 131I and injected intraperiton ealy. The 131I-AFP-R-LCA McAb was gathered in tumor of nude mice bearing human hepatocellular carcinoma (HCC). The tumor/liver radioactivity redio was 5.2, but normal mice IgG labeled by 131I-(131-mIgG) or free 131I was not gathered in tumor and distributed everywhere in average. The group of 131I-AFP-R-LCA McAb was positively visualized by gamma camea. The test showed that 131I-AFP-R-LCA McAb cured the nude mice bearing HCC. The tumor inhibition rate in 131I-AFP-R-LCA McAb group at the 4th week after treatment was significantly higher than those of the other groups (85%, 45%, 20%, 7% respectively, P < 0.05). The result showed that the AFP-R-LCA McAb have strong affinity and special combination to HCC cells and it can be recognized as a carrier for radioimmunodetection and radioimmunotherapy.
抗人甲胎蛋白变异体单克隆抗体（AFP-R-LCA McAb）用131I标记后腹腔注射。131I-AFP-R-LCA McAb聚集在荷人肝癌（HCC）裸鼠的肿瘤中。肿瘤/肝脏放射性比值为5.2，而用131I标记的正常小鼠IgG（131-mIgG）或游离131I未聚集在肿瘤中，而是平均分布于各处。131I-AFP-R-LCA McAb组经γ相机呈阳性显影。试验表明，131I-AFP-R-LCA McAb可治愈荷HCC裸鼠。治疗后第4周，131I-AFP-R-LCA McAb组的肿瘤抑制率显著高于其他组（分别为85%、45%、20%、7%，P<0.05）。结果表明，AFP-R-LCA McAb对HCC细胞具有强亲和力和特异性结合，可作为放射免疫检测和放射免疫治疗的载体。
