Vagnini M, Pitzurra L, Cartechini L, Miliani C, Brunetti B G, Sgamellotti A
Centro SMAArt, Dipartimento di Chimica, Università di Perugia, via Elce di Sotto 8, 06123, Perugia, Italy.
Anal Bioanal Chem. 2008 Sep;392(1-2):57-64. doi: 10.1007/s00216-008-2041-9. Epub 2008 Apr 8.
Immunofluorescence microscopy offers a highly specific analytical tool for unambiguous recognition and mapping of proteins in complex matrices. In the present work, the analytical potentials of immunofluorescence microscopy have been exploited to provide recognition of proteinaceous binders in painting cross-sections. An optimised analytical protocol is proposed for the identification of ovalbumin and of bovine serum albumin as markers of egg white and casein, respectively. The study has been carried out on laboratory model samples simulating both easel and mural paintings. The obtained results demonstrated the effectiveness of the method, suggesting the potential future use of immunofluorescence microscopy as a routine diagnostic tool in conservation science. Possible developments of the proposed methodology in order to improve the specificity of the method and its detection sensitivity are presented and discussed.
免疫荧光显微镜提供了一种高度特异性的分析工具，用于在复杂基质中明确识别和定位蛋白质。在本工作中，已利用免疫荧光显微镜的分析潜力来识别绘画横截面中的蛋白质结合剂。提出了一种优化的分析方案，用于分别鉴定作为蛋清和酪蛋白标志物的卵清蛋白和牛血清白蛋白。该研究在模拟架上画和壁画的实验室模型样品上进行。所得结果证明了该方法的有效性，表明免疫荧光显微镜未来有可能作为保护科学中的常规诊断工具。本文介绍并讨论了为提高该方法的特异性及其检测灵敏度而对所提出方法可能进行的改进。
