Hu Wenjing, Zhang Hui, Zhang Bingjian
1Department of Chemistry,Zhejiang University,Hangzhou 310027,P.R. China.
2Department of Cultural Heritage and Museology,Zhejiang University,Hangzhou 310028,P.R. China.
Microsc Microanal. 2015 Oct;21(5):1278-87. doi: 10.1017/S1431927615015147.
The identification and localization of organic binders in artworks are big challenges in archaeology and conservation science. Immunological techniques, such as enzyme-linked immunosorbent assay (ELISA) and immunofluorescence microscopy (IFM) have the potential to become powerful tools for the analysis of organic materials in ancient samples. In this study, ELISA and IFM techniques were combined to identify chicken ovalbumin, glue from several mammalian species, bovine milk, and fish glue in ancient Chinese painting samples. As binders, egg ovalbumin was found in two painting samples and animal glue was found in three samples, which were dated from the 4th to 8th centuries. The results clearly demonstrate that ELISA and IFM can be used to validate results from ancient samples.
艺术品中有机粘合剂的识别与定位是考古学和文物保护科学领域的重大挑战。免疫技术,如酶联免疫吸附测定(ELISA)和免疫荧光显微镜(IFM),有潜力成为分析古代样本中有机材料的强大工具。在本研究中,将ELISA和IFM技术相结合,以识别中国古代绘画样本中的鸡卵白蛋白、几种哺乳动物的胶水、牛奶和鱼胶。作为粘合剂,在两个绘画样本中发现了卵白蛋白,在三个可追溯到公元4至8世纪的样本中发现了动物胶。结果清楚地表明,ELISA和IFM可用于验证古代样本的结果。