基于转座子的细菌必需基因鉴定策略。

Transposon-based strategies for the identification of essential bacterial genes.

作者信息

Reznikoff William S, Winterberg Kelly M

机构信息

Marine Biological Laboratory, Bay Paul Center for Comparative Molecular Biology and Evolution, Woods Hole, MA, USA.

出版信息

Methods Mol Biol. 2008;416:13-26. doi: 10.1007/978-1-59745-321-9_2.

DOI:10.1007/978-1-59745-321-9_2

PMID:18392958

Abstract

We present a conceptual review of transposition-based strategies for determining gene essentiality on a one-by-one basis in bacteria. Many of the techniques are described in greater detail in individual chapters of this volume. The second section of this chapter deals with transposition-deletion-based strategies for determining the essentiality of blocks of genes. This latter approach has the potential to experimentally define the minimal required genome for a given organism.

摘要

我们对基于转座的策略进行了概念性综述，这些策略用于逐个确定细菌中的基因必需性。本卷的各章节对许多技术进行了更详细的描述。本章的第二部分讨论了基于转座缺失的策略，用于确定基因簇的必需性。后一种方法有可能通过实验确定给定生物体所需的最小基因组。