Giannattasio Sergio, Atlante Anna, Antonacci Lucia, Guaragnella Nicoletta, Lattanzio Paolo, Passarella Salvatore, Marra Ersilia
CNR, Istituto di Biomembrane e Bioenergetica, I-70126 Bari, and Trani (BA), Italy.
FEBS Lett. 2008 Apr 30;582(10):1519-25. doi: 10.1016/j.febslet.2008.03.048. Epub 2008 Apr 7.
To gain insight into the processes by which acetic acid-induced programmed cell death (AA-PCD) takes place in yeast, we investigated both cytochrome c release from yeast mitochondria and mitochondrial coupling over the time course of AA-PCD. We show that the majority of cytochrome c release occurs early in AA-PCD from intact coupled mitochondria which undergo only gradual impairment. The released cytochrome c can be reduced both by ascorbate and by superoxide anion and in turn be oxidized via cytochrome c oxidase, thus working both as a ROS scavenger and a respiratory substrate. Late in AA-PCD, the released cytochrome c is degraded.
为深入了解酵母中乙酸诱导的程序性细胞死亡（AA-PCD）发生的过程，我们研究了在AA-PCD过程中细胞色素c从酵母线粒体的释放以及线粒体偶联情况。我们发现，细胞色素c的大部分释放发生在AA-PCD早期，此时完整的偶联线粒体仅逐渐受损。释放的细胞色素c既能被抗坏血酸还原，也能被超氧阴离子还原，进而通过细胞色素c氧化酶被氧化，因此它既作为活性氧清除剂又作为呼吸底物发挥作用。在AA-PCD后期，释放的细胞色素c会被降解。
