酵母醋酸诱导的程序性细胞死亡可以在没有细胞色素 c 释放的情况下发生，而细胞色素 c 释放需要半胱天冬酶 YCA1。
Yeast acetic acid-induced programmed cell death can occur without cytochrome c release which requires metacaspase YCA1.
机构信息
CNR, Istituto di Biomembrane e Bioenergetica, Bari, Italy.
出版信息
FEBS Lett. 2010 Jan 4;584(1):224-8. doi: 10.1016/j.febslet.2009.11.072.
To investigate the role of cytochrome c (cyt c) release in yeast acetic acid-induced programmed cell death (AA-PCD), wild type (wt) and cells lacking metacaspase (Deltayca1), cytochrome c (Deltacyc1,7) and both (Deltacyc1,7Deltayca1) were compared for AA-PCD occurrence, hydrogen peroxide (H(2)O(2)) production and caspase activity. AA-PCD occurs in Deltacyc1,7 and Deltacyc1,7Deltayca1 cells slower than in wt, but similar to that in Deltayca1 cells, in which no cytochrome c release occurs. Both H(2)O(2) production and caspase activation occur in these cells with early and extra-activation in Deltacyc1,7 cells. We conclude that alternative death pathways can be activated in yeast AA-PCD, one dependent on cyt c release, which requires YCA1, and the other(s) independent on it.
为了研究细胞色素 c（cyt c）释放在酵母乙酸诱导的程序性细胞死亡（AA-PCD）中的作用，比较了野生型（wt）和缺乏介体型半胱天冬酶（Deltayca1）、细胞色素 c（Deltacyc1,7）以及两者都缺失（Deltacyc1,7Deltayca1）的酵母细胞中 AA-PCD 的发生、过氧化氢（H2O2）的产生和半胱天冬酶活性。与 wt 细胞相比，Deltacyc1,7 和 Deltacyc1,7Deltayca1 细胞中的 AA-PCD 发生较慢，但与不发生细胞色素 c 释放的 Deltayca1 细胞相似。这些细胞中均发生 H2O2 的产生和半胱天冬酶的激活，且在 Deltacyc1,7 细胞中早期和过度激活。我们得出结论，酵母 AA-PCD 中可以激活替代的死亡途径，一种依赖于细胞色素 c 的释放，这需要 YCA1，另一种途径不依赖于它。
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