Fitzpatrick Kate L, Tyerman Stephen D, Kaiser Brent N
School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus Urrbrae, SA 5064, Australia.
FEBS Lett. 2008 Apr 30;582(10):1508-13. doi: 10.1016/j.febslet.2008.03.045. Epub 2008 Apr 7.
Molybdenum is an essential micronutrient required by plants. The mechanism of molybdenum uptake in plants is poorly understood, however, evidence has suggested that sulfate transporters may be involved. The sulfate transporter from Stylosanthes hamata, SHST1, restored growth of the sulfate transport yeast mutant, YSD1, on media containing low amounts of molybdate. Kinetic analysis using 99MoO4(2-) demonstrated that SHST1 enhanced the uptake of molybdate into yeast cells at nM concentrations. Uptake was not inhibited by sulfate, but sulfate transport via SHST1 was reduced with molybdate. These results are the first measurement of molybdate transport by a characterised plant sulfate transport protein.
钼是植物所需的一种必需微量营养素。然而，人们对植物吸收钼的机制了解甚少，不过有证据表明硫酸盐转运蛋白可能参与其中。来自热带柱花草的硫酸盐转运蛋白SHST1，能使硫酸盐转运酵母突变体YSD1在含有少量钼酸盐的培养基上恢复生长。使用⁹⁹MoO₄²⁻进行的动力学分析表明，SHST1在纳摩尔浓度下增强了钼酸盐进入酵母细胞的吸收。吸收不受硫酸盐抑制，但通过SHST1的硫酸盐转运随着钼酸盐而减少。这些结果是首次对一种已鉴定的植物硫酸盐转运蛋白进行钼酸盐转运的测量。
