Khatri Kapil, Goyal Amit K, Gupta Prem N, Mishra Neeraj, Mehta Abhinav, Vyas Suresh P
Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Dr. Harisingh Gour Vishwavidyalaya, Sagar 470003, M.P., India.
Vaccine. 2008 Apr 24;26(18):2225-33. doi: 10.1016/j.vaccine.2008.02.058. Epub 2008 Mar 17.
The aim of the present work was to investigate the potential utility of glycol chitosan coated liposomes as nasal vaccine delivery vehicle for eliciting viral specific humoral mucosal and cellular immune responses. Plasmid pRc/CMV-HBs(S) encapsulated liposomes were prepared by dehydration-rehydration method and subsequently coated with glycol chitosan by simple incubation method. Liposomes were then characterized for their size, surface charge, entrapment efficiency, and ability to protect encapsulated DNA against nuclease digestion and for their mucoadhesiveness. The liposomes were then administered to mice in order to study their feasibility as nasal vaccine carriers. The developed liposomes possessed +9.8 mV zeta potential and an average vesicle size less than 1 microm and entrapment efficiency of approximately 53%. Following intranasal administration, glycol chitosan coated liposomes elicited humoral mucosal and cellular immune responses that were significant as compared to naked DNA justifying the potential advantage of mucosal vaccination in the production of local antibodies at the sites where pathogens enters the body.
本研究的目的是探讨壳聚糖二醇包被脂质体作为鼻腔疫苗递送载体，引发病毒特异性体液、黏膜和细胞免疫反应的潜在效用。采用脱水-复水法制备包裹质粒pRc/CMV-HBs(S)的脂质体，随后通过简单孵育法用壳聚糖二醇进行包被。然后对脂质体的大小、表面电荷、包封率、保护包裹的DNA免受核酸酶消化的能力及其黏膜黏附性进行表征。然后将脂质体给予小鼠，以研究其作为鼻腔疫苗载体的可行性。所制备的脂质体的ζ电位为+9.8 mV，平均囊泡大小小于1微米，包封率约为53%。鼻内给药后，壳聚糖二醇包被的脂质体引发了体液、黏膜和细胞免疫反应，与裸DNA相比具有显著性，证明了黏膜疫苗接种在病原体进入人体部位产生局部抗体方面的潜在优势。
