Robertson M W, Mehl V S, Richards M L, Liu F T
Medical Biology Institute, La Jolla, Calif.
Int Arch Allergy Appl Immunol. 1991;96(4):289-95. doi: 10.1159/000235511.
Multiple mRNA species encoding several predicted forms of the high-affinity IgE receptor alpha subunit (Fc epsilon RI-alpha) have been previously characterized from rat basophilic leukemia cells. Using the polymerase chain reaction procedure, we have extended these findings to show that one Fc epsilon RI-alpha mRNA variant, characterized by a 163-bp deletion within the coding sequence, exists in normal rat connective tissue mast cells as well as in both transformed and non transformed murine mast cell lines. In addition, a partial murine Fc epsilon RI-alpha genomic clone, spanning the internal-deletion sequence, has been identified, and from analysis of this sequence a mechanism of alternative pre-mRNA splicing is proposed. Finally, mRNA variants have been translated in a cell-free system and the protein products partially characterized.
先前已从大鼠嗜碱性白血病细胞中鉴定出多种编码高亲和力IgE受体α亚基（FcεRI-α）几种预测形式的mRNA种类。利用聚合酶链反应程序，我们扩展了这些发现，结果表明，一种FcεRI-α mRNA变体（其特征是编码序列内有163 bp的缺失）存在于正常大鼠结缔组织肥大细胞以及转化和未转化的小鼠肥大细胞系中。此外，已鉴定出一个跨越内部缺失序列的部分小鼠FcεRI-α基因组克隆，并根据该序列分析提出了一种可变前体mRNA剪接机制。最后，mRNA变体已在无细胞系统中进行翻译，并对蛋白质产物进行了部分表征。
