耐甲氧西林金黄色葡萄球菌的溶原性及原噬菌体在接合和非接合质粒转移中的作用。

Lysogeny of methicillin-resistant Staphylococcus aureus and the role of prophages in transfer of conjugative and non-conjugative plasmids.

作者信息

Zueva V S, Nesterenko L N, Dmitrenko O A, Akatov A K

机构信息

Laboratory of Staphylococcal Infections, Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, USSR.

出版信息

J Chemother. 1991 Oct;3(5):279-82. doi: 10.1080/1120009x.1991.11739106.

DOI:10.1080/1120009x.1991.11739106

PMID:1839741

Abstract

The lysogenicity of 49 strains of methicillin-resistant S. aureus (MRSA) isolated in Moscow clinics in the 1970s and '80s was studied by the method of mitomycin C induction. It was found that one strain had phage of serogroup B, 33 strains had serogroup F phages and 15 strains had phages of both serogroups. In the course of genetic crossing on nitrocellulose filters it was demonstrated that serogroups B and F prophages contained in recipient cells 1) increase the frequency of transfer of conjugative plasmid pG873 and 2) mobilize transfer of non-conjugative plasmids pE994 and rms7.

摘要

采用丝裂霉素C诱导法，对20世纪70年代和80年代在莫斯科诊所分离出的49株耐甲氧西林金黄色葡萄球菌（MRSA）的溶原性进行了研究。结果发现，一株带有B血清群噬菌体，33株带有F血清群噬菌体，15株同时带有这两种血清群的噬菌体。在硝酸纤维素滤膜上进行基因杂交的过程中发现，受体细胞中所含的B和F血清群原噬菌体：1）提高了接合质粒pG873的转移频率；2）促使非接合质粒pE994和rms7的转移。