流式细胞术免疫表型分析在恶性淋巴瘤诊断中的应用
[Application of flow cytometric immunophenotypic analysis in the diagnosis of malignant lymphoma].
作者信息
Ren Li-Qun, Xue Li-Yan, Bi Rui, Liang Jian-Ming, Lin Dong-Mei, Ma Jie, Lü Ning
机构信息
Department of Pathology, Cancer Hospital/Institute, CAMS & PUMC, Beijing 100021, China.
出版信息
Zhonghua Xue Ye Xue Za Zhi. 2007 Oct;28(10):671-6.
OBJECTIVE
To explore the value of flow cytometric immunophenotyping (FCI) in the diagnosis and differentiated diagnosis of lymphoma and explain the immunophenotypic features and differences of malignant lymphoma.
METHODS
Seventy four fresh samples of suspicious lymphoma were collected from Nov. 2004 to Aug. 2006. Each sample was individually evaluated by FCI. The results were analyzed and compared with the histopathological diagnosis.
RESULTS
Among the 74 cases, the FCI data consisted with the final morphological diagnosis in 61 cases (82.4%). For the diagnosis of B and T non-Hodgkin's lymphoma (NHL), thymoma, carcinoma and benign lesions of lymph node, the concordance between FCI data and morphological diagnosis were 93.5%, 100%, 100%, 100% and 81.3%, respectively.
CONCLUSION
Multi-parameter FCI analysis can provide important information and help for diagnosis of lymphoma. It is an assistant but necessary approach for the diagnosis and differential diagnosis of lymphoma.
目的
探讨流式细胞术免疫表型分析（FCI）在淋巴瘤诊断及鉴别诊断中的价值，并阐述恶性淋巴瘤的免疫表型特征及差异。
方法
收集2004年11月至2006年8月间74例可疑淋巴瘤新鲜标本。每例标本均采用FCI进行单独评估。对结果进行分析并与组织病理学诊断结果相比较。
结果
74例病例中，61例（82.4%）FCI数据与最终形态学诊断结果相符。对于B和T非霍奇金淋巴瘤（NHL）、胸腺瘤、癌及淋巴结良性病变的诊断，FCI数据与形态学诊断结果的一致性分别为93.5%、100%、100%、100%和81.3%。
结论
多参数FCI分析可为淋巴瘤诊断提供重要信息及帮助。它是淋巴瘤诊断及鉴别诊断的辅助但必要的方法。
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