Stöveken Tim, Steinbüchel Alexander
Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstr. 3, 48149 Münster, Germany.
Angew Chem Int Ed Engl. 2008;47(20):3688-94. doi: 10.1002/anie.200705265.
Bacterial acyltransferases are a new class of enzymes, and the first member was identified as WS/DGAT in Acinetobacter baylyi ADP1. Their unspecificity have been used in several biotechnological applications for lipid modification, a field that has been dominated by the use of lipases. Examples are the biosynthesis of jojoba-like wax esters and fatty-acid ethyl esters. In addition, these enzymes are also capable of synthesizing acylthioesters. Acyloxoesters and acylthioesters can thus be produced in vivo by whole-cell fermentations rather than in vitro in an enzyme reactor. In this Minireview, we focus on the biotechnological utilization of acyltransferases for the production of modified lipids from renewable resources.
细菌酰基转移酶是一类新型酶，其首个成员在鲍氏不动杆菌ADP1中被鉴定为WS/DGAT。它们的非特异性已被用于脂质修饰的多种生物技术应用中，而该领域一直由脂肪酶的使用主导。例如霍霍巴样蜡酯和脂肪酸乙酯的生物合成。此外，这些酶还能够合成酰基硫酯。因此，酰氧基酯和酰基硫酯可以通过全细胞发酵在体内而非在酶反应器中体外生产。在本综述中，我们重点关注酰基转移酶在利用可再生资源生产改性脂质方面的生物技术应用。
